Characterization of Green Pigment from Sauropus androgynus Shoot Cultures

The dark green leaves of Sauropus androgynus (Euphorbiaceae) have various nutritive values and are commonly used for human consumption as food, medicine, natural dye substance in South-east Asia. Shoot cultures this plant were established by adding concentrations kinetin (Kn) benzyl adenine (BA) using nodal explants. best results recorded when Kn 0.1 mg/L was with BA 1 (BA1Kn0.1). Spectrophotometric analysis showed two peaks pigment shoot cultures, A (λmax = 663.6 - 663.8 nm, absorbance 0.1111) B (λmax= 611.3 613.9 0.0390). Thin Layer Chromatography (TLC) spots (Rf Y 0.31 Rf Z 0.25) on medium supplemented BA1Kn0.1 10 days. Pigment profiles culltures similar to their corresponding mother plants. Random Amplified Polymorphic DNA (RAPD) a preliminary technique evaluate the genetic similarity It four banding patterns leaves, ranging from 271-765 bp.